This study was carried out with an objective to compare the cardiovascular fitness of female students of Department of Physical Education, Panjab University, Chandigarh and female students of Government College of Yoga, Sector-23 Chandigarh within the age group of 20 to 25years. The parameter include Physical Fitness Index, Modified Harvard-Step test on sample of 50 female students. To compare the main difference t-test was employed with 0.05 level of significance. The result of the study depicts that the students of Department of Physical Education, Panjab University Chandigarh are found superior than students of Government College of Yoga, Sector-23 Chandigarh on Cardio-vascular fitness. The calculated ‘t’ value 4.138 was found significant at 0.05 level against the tabulated value 2.02 in favor of female students of Department of Physical Education, Panjab University Chandigarh.