The purpose of the study was to analysis the relative importance of Bio-Motor Variables among women Volleyball players. To achieve this purpose of the study one hundred and twenty (N=120) women Volleyball were selected. Among them, forty Spikes (n=40), Setters (n=40) and All-rounder’s women Volleyball from KSWU Universities who had participated in the Inter-collegiate Volleyball tournament during the year 2014-2015 were selected as subjects. The age of the subjects were ranged from 18 to 24 years. Among the Bio-Motor Variables only Speed selected as criterion variable. Speed was measured by 30 Meters Run Test. The data collected from the three groups was statistically analyzed by using Analysis of variance (ANOVA). Scheffe’s post hoc test was applied to determine the significant difference between the paired means. In all the cases 0.05 level of confidence was fixed significance was fixed. The results of the study showed that there was a significant difference among Spikes, Setters and All-rounder’s.