Hamstring flexibility plays an important role in basic movements such as walking and running. Studies have been suggested that hamstring tightness are associated with various sports injuries. This study is done to find out the effectiveness of retro walking on hamstring tightness and dynamic balance in young collegiate students. Experimental study design.40 samples participated in the study with the age group of 17-25 years.40 subjects were divided into two groups, Group A [Retrowalking and active stretching] and Group B [Active stretching].Pre and post test values of AKET and SEBT of Group A and Group B were measured. Total duration of the study was for 4 weeks. Active knee extension test and star excursion balance test was used as the outcome measure. Both groups have yielded significant improvements on the hamstring flexibility (P=0.000).Dynamic balance was significantly improved in all 8 directions in Group A with (P=0.000) and Group B showed significant improvement in 7 directions with (P=0.000) in medial, antero-medial, posterior, posteromedial, postero-lateral directions followed by lateral (P=.004),antero-lateral(P=.001). And insignificant improvement in anterior direction (P=0.008). This study concludes that in Group A (RW AND AS) and Group B (AS) there is improvement in hamstring flexibility. In post intervention AKET between two groups, Group A showed highly significant improvement than Group B. In post intervention SEBT between two groups, Group A (RW and AS) showed highly significant improvement than Group B (AS) in four directions such as medial, antero-lateral, posterior, postero-lateral and insignificant improvement in other four directions such as lateral, anterior, antero-medial, postero-medial.