The aim of the present study was to compare the physical fitness parameters of the rural and urban boys from Punjab. For the purpose of this study, 60 children (30 rural and 30 urban) of 14 years age group were selected to participate in the study. All the children were measured for various physical fitness parameters. Explosive strength was assessed with the help of standing broad jump and standing vertical jump. The speed ability was measured with the help of 30 meter sprint (Flying Start). The endurance of the rural and urban children was assessed with the help of 800 meter run. Sit and reach test was used to assess the flexibility of the children. The grip strength was measured with the help of hand dynamometer. The medicine ball put was used to measure the strength of arms. The independent samples t-test revealed that the rural boys were found to have significantly better performance on speed (p<0.05), medicine ball put (p<0.05), standing broad jump (p<0.05), and grip strength of both right (p<0.05) and left (p<0.05) hands as compared to the urban boys. It can be concluded that the rural boys had better scores on physical fitness parameters as compared to urban boys.